Acceleration of Callus Formation in Date Palm (Phoenix dactylifera L.) Zygotic Embryos

Mohanned Abdalla Ali Abdalla, Abdel Gaffer Elhag Said

Abstract


This study was carried out to test the effect of water soaking of
seeds of date palm ( P. dactylifera L.) as a pre-empryo excision treatment,
on in vitro culture of zygotic empryos. The objective was optimization of
organogenic callus formation. Mature seeds of “Barakawi” and “Mishrig
Wad Laggi” date palm cultivars were used as sources of embryos.
Durations of 0- up to 9-days of seed water soaking, prior to embryo
excision and culture, were tested. Murashige and Skoog (1962) medium
was used as basal medium. The cultures responded positively to the water
soaking treatments and resulted in the organogenic responses of swelling,
germination, callus and adventitious roots formation were obtained. The
magnitude and type of response varied with cultivar and soaking duration.
All water soaking treatments increased the measured responses relative to
the control. The results showed that a 6-day soaking treatment was
optimal for all measured responses compared with the other periods of
soaking tested. Calli formation in 6-day water soaking treatment were
50% and 70% for “Barakawi” and “Mishrig Wad Laggai” ,respectively.
However, increasing the soaking duration beyond the 6-day had a marked
negative effect on morphogenic response regardless of cultivar. The best
results for all measured parameters were obtained with embryos of
“Mishrig Wad Laggi” compared with embryos excised from “Barakawi”.
It seems that soaking date palm seeds in water prior to embryo excision
play an important role, in enhancing morphogenic response and callus
formation in date palm tissue culture

Keywords


Date palm; callus formation; Phoenix dactylifera L.; water soaking

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References


Abdullah, G. and Maroff, A. (2007). The influence of a few gibberellins

levels on the seed germination and the growth of seedlings of three

varieties of date palm (Phoenix dactylifera L.) Proceedings of the

International Conference on the Technology of Horticultural

Productivity for Sustainable Development and Biodiversity. Aleppo,

Syria. Pp. 204-215.

Ahmed, H.S. (1986). Endogenous growth substances of date palm seeds

and their relation to germination. Proceedings of the Second

Symposium on the Date Palm in Saudi Arabia. Volume 1. pp 173-

Al-Wasel, A.S. and Warrag, M.O.A. (1998). Effects of fruit

developmental stage, seed scarification and operculum removal on

seed germination of date palm. Journal of King Saud University,

Agricultural Science, 10,153-161.

Bekheet, S.A.; Taha, H.S.; Hanafy, M.S. and Solliman. M.S. (2008).

Morphogenesis of sexual embryos of date palm cultured in vitro and

early identification of sex type. Journal of Applied Science and

Research, 4, 345- 352.

Bhansali, R.; Kauk, R. and Dass. H. (1988). Mass cloning of date palm

plantlets through repetitive somatic embryogenesis. Journal of Plant

Anatomy and Morphology. 5, 73-79.

Bonga, J. M. (1982). Vegetative propagation in relation to juvenility,

maturity and rejuvenation. In: J.M. Bonga, and D.J.Durzan, (Eds.).

Tissue Culture in Forestry, Martinus Nijhoff/ DR D. Junk

Publishers, The Hague, Pp. 387- 412

Carpenter, W.J. and Maekawa, S. (1991). Substrate moisture level

governs the germination of verbena seed. HortScience, 26, 1469-

Chee, P.P. (1994). In vitro culture of zygotic embryos of Taxus species.

HortScience, 29,695-697.

Compton. M. E. and Gray. D. J. (1994). Adventitious shoot organogenesis

and plant regeneration from cotyledons of tetraploid watermelon.

HortScience, 29, 211-213.

Eshraghi, P.; Zarghami, R. and Mirabdulbaghi, M. (2005). Somatic

embryogenesis in two Iranian date palm cultivars. African Journal

of Biotechnology, 4,1309-1312.

Finnie, J. F.; Ackermann, C. and vanStaden, J. (1989). In vitro culture of

guayule using pretreated seeds. HortScience, 24,836-837.

Gabr, M. F. and Tisserat. B. (1985). Propagating palms in vitro with

special emphasis on the date palm (Phoenix dactylifera L.). Scientia

Horticulturae, 25, 255-262.

Hussey, G. (1978). The application of tissue culture to the vegetative

propagation of plants. Scientific Progress Oxford, 65, 185-208.

Jain, S. M. (2007). Recent advances in date palm tissue culture and

mutagenesis. Acta Horticulturae, 736, 205-211.

Janick, J. and Whipkey, A. (1986). In vitro propagation of Cuphea

wrightii. HortScience, 21(1), 135-137.

Litz, R. E.; Moor, G. A. and Srinivasan, C. (1985). In vitro system for

propagation and improvement of tropical fruits and palms.

Horticultural Review, 7, 157-200.

Maekawa, S. and Carpenter, W. J. (1991). Verbena seed hilum

morphology contributes to irregular germination. HortScience, 26,

-132.

Murashige, T. (1974). Plant propagation through tissue culture. Annual

Review of Plant physiology, 25, 135-166.

Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth

and bioassays with tobacco tissue cultures. Physiologia Plantarum,

, 473-479.

Nixon, R.W. and Carpenter, J, B. (1978). Growing Dates in the United

States. USDA Agric. Inf. Bulletin. 207, Washington, D C.

Nwankwo, B.A. and Krikorian, A.D. (1983). Morphogenetic potential of

embryo and seedling-derived callus of Elaeis guineensis Jacq var.

pisifera Becc. Annals of Botany, 51, 65-76.

Olumekun, V.O. and Remison, S.U. (1985). The effects of soaking, heat

treatment and growth regulators on the germination of date palm

(Phoenix dactylifera L.). Journal of the Nigerian Institute for Oil

Palm Research, 7,107-119.

Omar, M.S. (1988). In vitro responses of various date palm explants. Date

Palm Journal, 6, 371-389.

Pierik, R.L.M. (1990). Rejuvenation and micropropagation. International

Association of Plant Tissue Culture Newsletter, 62, 11-21.

Rao, A.M.; Reddy, S.K.; Rao, V.R.M. and Bhalla, J.K. (2001). Clonal

propagation of date palm through somatic embryogenesis. Indian

Forester, 127, 463-465.

Reynolds, J. F. and Murashige, T. (1979). Asexual embryogenesis in

callus culture of palms. In Vitro, 15,383-387.

Sharma, D.R.; Kumari, R. and Chowdhury, J.B. (1980). In vitro culture of

female date palm (Phoenix dactylifera L.) tissues. Euphytica, 29,

-174

Sharma, D.R.; Deepak, S.; and Showdhury, J.B. (1986). Regeneration of

plantlets from somatic tissues of the date palm, Phoenix dactylifera

Linn. Indian Journal of Experimental Biology, 24,763-766.

Sidky, R.A. and Zaid, Z.E. (2011). Direct production of somatic embryos

and plant regeneration using TDZ and CPPU of date palm (Phoenix

dactylifera L.). International Journal of Academic Research, 3, 792-

Srinivasan, C.; Litz, R.E.; Barker, J. and Norstog, K. (1985). Somatic

embryogenesis and plantlet formation from Christmas palm callus.

HortScience, 20,278-280.

Tisserat, B. (1981). Date Palm Tissue Culture. U.S. Department of

Agriculture, Agricultural Research Service, Western Region.

Advanced Agricultural Technology. AAT-W-17, 1-50.

Tisserat, B. (1982). Factors involved in the production of plantlets from

date palm callus cultures. Euphytica, 31, 201-214.

Tisserat, B. (1984). Propagation of date palms by shoot tip culture.

HortScience, 19, 230-231.

Tisserat, B. and DeMason, D.A. (1980). A histological study of the

development of embryos in organ cultures of Phoenix dactylifera L.

Annals of Botany, 16,465-742.

Veramendi, J. and Navarro, L. (1997). Influence of explant source of

adult date palm (Phoenix dactylifera L.) on embryogenic callus

formation. Journal of Horticultural Science, 72, 655-671.

Zaid, A. and Tisserat, B. (1983). In vitro shoot tip differentiation in

Phoenix dactylifera L. Date Palm Journal, 2, 163-182.

Zaid, A. and Tisserat, B. (1984). Survey of the morphogenetic potential of

excised palm embryos in vitro. Crop Research (Horticultural

Research), 24, 1-9.


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