Propagation of Pineapple by Shoot Tip Culture

Abstract

This study was conducted with the objective of modifying the
composition of MS medium for the clonal propagation of pineapple using
shoot tips of "Smooth Cayenne" cultivar. Modifications were made on
various medium components. Results showed that both MS salts at the
full or half strength were optimal, and there was no significant difference
between them. Sucrose concentrations of 3% and 6% were better than
other concentrations tested for growth and development of plantlets. The
cultures responded positively to the increase of adenine sulfate and 80
mg/l was the optimal. As for the additions of NAA and BA, alone or in
combinations, the best results were recorded with the combination of
NAA at 0.01 mg/l, and BA at 3.0 mg/l where the largest number of shoots
was obtained. Better explants performance was achieved on liquid
medium with cotton support compared to solid medium.