DETECTION OF ANTIBIOTIC RESIDUES IN TABLE EGGS USING DISC ASSAY AND PREMI TEST IN KHARTOUM STATE, SUDAN

Abstract

The aim of this study was to detect antibiotic residues in table eggs
consumed in Khartoum State- Sudan. A total of 157 chicken egg samples
were collected from 32 poultry farms and 74 sale points. A structured
questionnaire was also used to gather information about the hygienic
status of the farms, common signs of diseases, awareness of farmers and
type of antibiotics used. It was found that diarrhea was present in 34.6%
of the farms and the most frequently used antibiotic was tetracycline in
36.4% of the farms. The farmers were supervising their own farms in
57.6% of the farms, and 94% of the farmers lack the knowledge about
withdrawal period.
The disc assay method and Premi test kit were used for antibiotic residues
detection. The total number of positive reactors was 49.6% and 12.7%
respectively. Using the Kappa statistic the agreement between the two
tests was 0.30 ( poor agreement) and the percent of the agreement was
61.7% .
The data showed the poor hygienic status of poultry farms with the
concomitant use of antibiotics for treatment of diseases and the lack of
knowledge regarding the use of antibiotics resulted in the high
percentage of positive samples. Although antibiotics were excessively
used in poultry farms the Premi test detected lower positive percentage of
the positive samples while the disc assay correlated well with this
excessive use of antibiotics