THERMOSTABILITY IMPROVEMENT OF A RECENTLY DEVELOPED LIVE ATTENUATED SUDANESE CAMELPOX VACCINE

Abstract

A sudanese camel pox (CMP) candidate vaccine recently developed was
subjected to gradual heating to improve its thermostability. Thermostability testing of the
vaccine revealed that at 56˚C all the formulations lost their titer quickly with more than
3 to 5 log disappeared only in 30 and 60 minutes of incubation, respectively. The virus
was then subjected to - heating at 56˚C for increasing time periods; the final passage was
then retested for thermostability. Thermostability retesting of the recovered virions
revealed that at 56˚C wild type virus and the attenuated candidate vaccines lost a titer of
- and - log10 CCID50 upon incubation for 30 and 60 minutes, respectively.Compared
with the original vaccine virus, a degree of thermostability improvement of the candidate
vaccinewas attained. The information gathered in this work showed that; it is possible for
the treated CP vaccine to have adequate short-term stability at non-freezing temperatures
to support manufacturing, short-term shipping and storage. Improving thermostability
should significantly enhance the utility of the vaccine during the field work for the
control of CMP outbreaks.