THERMOSTABILITY IMPROVEMENT OF A RECENTLY DEVELOPED LIVE ATTENUATED SUDANESE CAMELPOX VACCINE

Maysaa A M, Abd-Ellatif M M, Hamdi Eljack, Ibrahim A A, Khalafalla AI

Abstract


A sudanese camel pox (CMP) candidate vaccine recently developed was subjected to gradual heating to improve its thermostability. Thermostability testing of the

vaccine revealed that at 56˚C all the formulations lost their titer quickly with more than 3 to 5 log disappeared only in 30 and 60 minutes of incubation, respectively. The virus was then subjected to - heating at 56˚C for increasing time periods; the final passage was then retested for thermostability. Thermostability retesting of the recovered virions revealed that at 56˚C wild type virus and the attenuated candidate vaccines lost a titer of

 

- and - log10 CCID50 upon incubation for 30 and 60 minutes, respectively.Compared with the original vaccine virus, a degree of thermostability improvement of the candidate vaccinewas attained. The information gathered in this work showed that; it is possible for the treated CP vaccine to have adequate short-term stability at non-freezing temperatures to support manufacturing, short-term shipping and storage. Improving thermostability should significantly enhance the utility of the vaccine during the field work for the control of CMP outbreaks.


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ISSN: 243456

U. of  K. J. Vet. Med. Anim. Prod.