In Vitro Propagation of Chrysanthemum morifolium Ramat Using Stem Nodal Explants

Abstract

This study was conducted to investigate the effect of some
growth regulators on in vitro shoot regeneration, multiplication and
rooting of Chrysanthemum morifolium to develop a protocol for its mass
propagation. Nodal explants of C. morifolium were cultured on MS basal
medium supplemented with the cytokinins BAP and kinetin, both at 0.0,
0.1, 0.2, 0.5, 1.0, 2.0, 5.0, 10.0 mg/l, for shoot regeneration. The
regenerated shoots were separated from the nodal explants and cultured
on MS medium supplemented with BAP and kinetin, both at 0.0, 0.1, 0.2,
0.5 mg/l for shoot multiplication. The shoots were then cultured on MS
medium supplemented with 0.0, 0.1, 0.2, 0.5 mg/l of IBA for rooting.
Twenty explants were used per treatment. The percentage of responded
explants, average number of shoots per explant, length of shoot,
percentage of rooted shoots, number of roots per shoot and length of root
were determined. The concentration of 0.1 mg/l of both BAP and kinetin
showed the best response with regard to shoot regeneration and
multiplication giving the highest number of shoots per explant.
Cytokinin–free medium gave significantly the highest length of shoot. All
IBA treatments gave 100% rooting. However, MS+ 0.5 mg/l IBA resulted
in significantly the highest number of roots per shoot, and IBA–free
medium resulted in significantly the highest root length