Propagation of Grape by Nodal Stem cuttings

Abstract

Experiments were conducted to test the effect of MS salts
concentration, potassium dihydrogen phosphate and growth regulators on
shoot proliferation and growth in grapevine (Vitis vinifera L.), using
explants derived from in vitro grown plantlets. Murashige and Skoog
original medium was used as basal medium. Shoot proliferation occurred
principally when using nodal stem segments as explants. Among MS-salt
strengths tested, full MS- strength (1X) was optimum for shoot
proliferation and elongation. Increasing the original phosphorus
concentration in the medium by the addition of 170 mg/L KH2 PO 4 had a
positive, marked effect on shoot number and shoot length. The nodal stem
segment explants responded best to relatively low concentrations of NAA
and kinetin, and a large number of shoots and good plantlets height were
obtained on the medium containing 0.1mg/L NAA and 1.0 mg/L kinetin.
Root formation occurred on the medium containing 0.3 mg/L IBA.