Assessment of the Genetic Diversity of Some Female Jojoba (Simmondsia chinensis (Link) Schneider) Genotypes Growing in the Semi-arid Highlands of Sudan using ISSR Markers

Authors

  • Ismail A. Mohammed Department of Botany and Agricultural Biotechnology, Faculty of Agriculture, University of Khartoum, Khartoum North, Shambat, P.O. Box 32, Postal Code 13314, Sudan
  • Rania S. Elsanousi Department of Botany and Agricultural Biotechnology, Faculty of Agriculture, University of Khartoum, Khartoum North, Shambat, P.O. Box 32, Postal Code 13314, Sudan
  • Lana A. Ibrahim Department of Botany and Agricultural Biotechnology, Faculty of Agriculture, University of Khartoum, Khartoum North, Shambat, P.O. Box 32, Postal Code 13314, Sudan.
  • Ramah A. AbdAlgadir Department of Botany and Agricultural Biotechnology, Faculty of Agriculture, University of Khartoum, Khartoum North, Shambat, P.O. Box 32, Postal Code 13314, Sudan.
  • Sayeda O. Elhoweiris Department of Botany and Agricultural Biotechnology, Faculty of Agriculture, University of Khartoum, Khartoum North, Shambat, P.O. Box 32, Postal Code 13314, Sudan.
  • Fatima M. Osman Department of Botany and Agricultural Biotechnology, Faculty of Agriculture, University of Khartoum, Khartoum North, Shambat, P.O. Box 32, Postal Code 13314, Sudan.
  • Seif M. Gasim Department of Agronomy, Faculty of Agriculture, University of Khartoum, Khartoum North, Shambat, P.O. Box 32, Postal Code 13314, Sudan.

DOI:

https://doi.org/10.53332/uofkjas.v29i1.4

Keywords:

jojoba, genetic diversity, liquid wax, ISSR marker, polymorphism, Sudan

Abstract

The knowledge of genetic diversity of a crop like Jojoba, a commercially grown plant to produce unique liquid oil from its seed, is essential in a breeding program aiming to the development of elite genotypes
for large-scale production. This study was carried out to evaluate the genetic diversity of 25 female jojoba genotypes growing in Arkaweet Area, East Sudan, using ISSR Markers. Genomic DNA was extracted from leaves of the
studied genotypes and quantified for PCR amplification. Five ISSR primers were used for PCR amplification to investigate the genetic diversity among the female jojoba genotypes. ISSR primers showed high polymorphic pattern and produced a total of 54 polymorphic bands with an average of 10.8 polymorphic bands per primer. The average of polymorphic information content (PIC) value of 0.37 indicates moderate polymorphism. The genetic
diversity ranged from 0.01 to 0.85. The results indicate the effectiveness of using ISSR molecular markers to reveal genetic variability among the studied female jojoba genotypes, a finding that could be used for development of
elite jojoba genotypes, which provide potential for large-scale jojoba production in Sudan.

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Published

2021-08-02

How to Cite

Mohammed, I. A., Elsanousi, R. S., Ibrahim, L. A., AbdAlgadir, R. A., Elhoweiris, S. O. ., Osman, . F. M., & Gasim, S. M. (2021). Assessment of the Genetic Diversity of Some Female Jojoba (Simmondsia chinensis (Link) Schneider) Genotypes Growing in the Semi-arid Highlands of Sudan using ISSR Markers. University of Khartoum Journal of Agricultural Sciences, 29(1). https://doi.org/10.53332/uofkjas.v29i1.4

Issue

Vol. 29 No. 1 (2021): University of Khartoum Journal of Agricultural Sciences

Section

Articles