CHEMICAL AND BIOCHEMICAL PROFILE OF FILAMENTOUS NON-TUBERCULOUS MYCOBACTERIA ISOLATED FROM CASEOUS LESIONS IN SUDAN

Abstract

The aims of this study are to isolate the possible mycobacterial causes of
caseated bovine tissues and subsequently identify the filamentous farcy
agents using chemotaxonomic profile. A total of 59 acid fast filaments
were isolated from bovine caseous lesions in South Darfur State. The
isolates were conventionally identified using cultural and microscopic
tests. For biochemical analysis 32 isolates were investigated while, the
chemical profile applied for 24 isolates. Toluene-Acetonitrile
precipitation method was applied to determine the quantitative mycolic
acid precipitation. Subsequently thin layer chromatography was done
from the precipitates for qualitative mycolates. Chloroform / methanol
method was applied for typing phenolic glycolipids.Rapidly growing
filamentous mycobacterial isolates (n = 32) were identified as M.
farcinogenes (n =26) and M. senegalense (n =6) by their biochemical
profiles, glycolipids and mycolic acid patterns. Mycolic acid analysis has
a reliable role in differentiation and taxonomy of Mycobacteria from
Nocardia and other related bacteria. However glycolipids test could
differentiate the farcy agents into M. farcinogenes and M. sengelenses.