Isolation and Genetic Characterization of Methicillin–Resistant Staphylococcus aureus (MRSA) from Animal Products in Khartoum State, Sudan

Authors

  • Alaa Khalil Unit of Animal Health and Safety of Animal Products, Institute for Studies and Promotion of Animal Exports, University of Khartoum, Khartoum North, Sudan
  • Alaa Gamar Unit of Animal Health and Safety of Animal Products, Institute for Studies and Promotion of Animal Exports, University of Khartoum, Khartoum North, Sudan, Faculty of Veterinary Science, West Kordofan University, Gibeish, Sudan
  • Elshima Elmahy Unit of Animal Health and Safety of Animal Products, Institute for Studies and Promotion of Animal Exports, University of Khartoum, Khartoum North, Sudan
  • Enass M. Abdalla Unit of Animal Health and Safety of Animal Products, Institute for Studies and Promotion of Animal Exports, University of Khartoum, Khartoum North, Sudan-Ministry of Agriculture, Irrigation and Animal Resources of North Darfur State, El Fashir, Sudan
  • Mashaer Fadul Unit of Animal Health and Safety of Animal Products, Institute for Studies and Promotion of Animal Exports, University of Khartoum, Khartoum North, Sudan - Ministry of Agriculture, Irrigation and Animal Resources of Khartoum State, Khartoum North, Sudan
  • Mohamed E. Mukhtar Department of Agricultural Extension and Rural Development, Faculty of Agriculture, University of Khartoum, Khartoum North, Sudan
  • Reem M. A. Elsanousi Department of Microbiology, College of Medicine, University of Bahri, Khartoum, Sudan
  • Mohamed T. Musa Department of Surgery, Faculty of Medicine, University of Khartoum, Khartoum, Sudan
  • Sharfi A. O. Ahmed ENT Department, Faculty of Medicine, Omdurman Islamic University, Omdurman, Sudan
  • Sulieman M. El Sanousi Department of Microbiology, Faculty of Veterinary Medicine, University of Khartoum, Khartoum North, Sudan
  • Kamal H. Eltom Unit of Animal Health and Safety of Animal Products, Institute for Studies and Promotion of Animal Exports, University of Khartoum, Khartoum North, Sudan

Keywords:

S. aureus, MRSA, mecA, mecC, PVL, hlg, milk, meat, chicken, camel, cattle

Abstract

The aim of this study was to investigate the presence of methicillin-resistant Staphylococcus aureus (MRSA) in animal products to contribute in establishing epidemiological data on antimicrobial resistance in the Sudan. Samples (N = 616) of animal products (milk, cheese, chicken and retail meat) were collected from different sources in Khartoum State and subjected to bacteriological and molecular biology techniques for isolation and characterization of S. aureus. S. aureus was isolated from 62 (10.1%) of the samples with frequencies of 32.7%, 23.3%, 21.5% 7.2% and 4.75% from cheese, chicken meat, retail meat, camel milk and cattle milk, respectively. Methicillin resistance was detected phenotypically among all (100%) cattle milk, 78.6% of retail meat, 71.4% of chicken meat, 50% of camel milk and 23.5% of cheese S.

aureus isolates. The methicillin resistance gene (mecA) was detected in 3 isolates with one being positive for mecA analogue (mecC) as well. mecA gene detected in these isolates belonged to none of S. aureus SCCmec (staphylococcal cassette chromosome mec) known types. Panton-Valentine Leuckocidin (PVL), haemolysin (hlg) and toxic shock syndrome toxin (tsst) genes were detected as 12.9%, 9.7% and 0.0%, respectively, in all S. aureus isolates. High prevalence of methicillin resistance among S. aureus isolates from animal products, especially from cattle milk indicates widespread of antimicrobial resistance and necessitates adoption of good animal production hygiene practices. The genes responsible for methicillin resistance among phenotypically MRSA but mecA/mecC-negative strains are yet to be identified.

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Published

2022-12-28

How to Cite

Khalil, A., Gamar, A. ., Elmahy, E., Abdalla, E. M. ., Fadul, M., Mukhtar, M. E., Elsanousi, R. M. A., Musa, M. T., Ahmed, S. A. O. ., El Sanousi, S. M. ., & Eltom, K. H. (2022). Isolation and Genetic Characterization of Methicillin–Resistant Staphylococcus aureus (MRSA) from Animal Products in Khartoum State, Sudan. Journal of Veterinary Medicine and Animal Production, 10. Retrieved from http://onlinejournals.uofk.edu/index.php/vet/article/view/1307

Issue

Vol. 10 (2019): U. of K. J. Vet. Med. Anim. Prod., Vol.10, Issue 1&2

Section

Articles