Application of Conventional Reverse Transcription (RT) Polymerase Chain Reaction (RT-PCR) for Detection of African Horse Sickness Virus Serogroup

Authors

  • Siham T. Karamalla Molecular Biology Laboratory (MBL), Department of Clinical Medicine, Faculty of Veterinary Medicine, University of Khartoum, P.O. Box 32 Khartoum North, Sudan.
  • Mawahib H. Eldegail Molecular Biology Laboratory (MBL), Department of Clinical Medicine, Faculty of Veterinary Medicine, University of Khartoum, P.O. Box 32 Khartoum North, Sudan.
  • Ahmed A. Gubran Molecular Biology Laboratory (MBL), Department of Clinical Medicine, Faculty of Veterinary Medicine, University of Khartoum, P.O. Box 32 Khartoum North, Sudan.
  • Khitma H. Elmalik Molecular Biology Laboratory (MBL), Department of Clinical Medicine, Faculty of Veterinary Medicine, University of Khartoum, P.O. Box 32 Khartoum North, Sudan.
  • Imadeldin E. Aradaib Molecular Biology Laboratory (MBL), Department of Clinical Medicine, Faculty of Veterinary Medicine, University of Khartoum, P.O. Box 32 Khartoum North, Sudan.

Keywords:

African horse sickness, virus cell culture, polymerase chain reaction

Abstract

A conventional reverse transcriptase (RT) polymerase chain reaction (RT-PCR) for detection of African Horse sickness virus serogroup in cell culture was developed and evaluated. The sensitivity of the assay indicated that the RT-PCR produced a 240- base pair (bp) PCR product from all 9 serotypes of AHSV propagated in cell cultures. The specificity of the assay indicated that the PCR products were not amplified when the RT-PCR was applied to RNA from other related orbiviruses including Blue tongue virus (BTV) serotype 1, 2, and 4; Epizootic hemorrhagic disease virus (EHDV) serotype 1 and 2; Sudanese isolates of palyam viruses and total nucleic acid extracts from uninfected Vero cell control. The African horse sickness virus RT-PCR assay should be used as a rapid method for detection of AHSV RNA in cell culture. In addition, the assay could be optimized for rapid detection of AHSV in clinical samples during an epizootic of the disease among susceptible equines

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Published

2023-01-12

How to Cite

Karamalla, S. T. ., Eldegail, M. H., Gubran, A. A., Elmalik , K. H., & Aradaib, I. E. (2023). Application of Conventional Reverse Transcription (RT) Polymerase Chain Reaction (RT-PCR) for Detection of African Horse Sickness Virus Serogroup. Journal of Veterinary Medicine and Animal Production, 11. Retrieved from http://onlinejournals.uofk.edu/index.php/vet/article/view/1361

Issue

Vol. 11 (2020): U. of K. J. Vet. Med. Anim. Prod., Vol.11, Issue 1&2

Section

Articles